AbstractMonitoring food safety is essential for protecting the health and safety of consumers. Conventional methods used are time consuming and laborious, requiring anywhere from three to seven days to obtain results. Thus, better monitoring methods are required. In this study, a laminated lab-on-paper chip was developed, and its use for the screening of ready-to-eat seafood was demonstrated. The assay on a chip was based on loop-mediated isothermal DNA amplification (LAMP) of the hly gene of Listeria monocytogenes and fluorescence signal detection via SYBR GoldTM. Overall assay processes were completed in 4.5 h., (including 3.5 h. incubation for the bacteria enrichment, direct DNA amplification with no DNA extraction, and signal detection), without relying on standard laboratory facilities. Only positive samples induced fluorescence signals on chip upon illumination with UV light (λ = 460). The method has a limit of detection of 100 copies of L. monocytogenes DNA per 50 g of sample. No cross-reactivity was observed in samples contaminated with other bacteria. On-site monitoring of the seafood products using this chip revealed that one of 30 products from low sanitation vendors (3.33%) were contaminated, and these agreed with the results of PCR. The results demonstrated a benefit of this chip assay for practical on-site monitoring. View Full-Text
Keywords: Lab-on-paper chip; Listeria monocytogenes; Detection; LAMP; hly gene; frozen seafoodLab-on-paper chip; Listeria monocytogenes; Detection; LAMP; hly gene; frozen seafood►▼ Figures
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Pisamayarom, K.; Suriyasomboon, A.; Chaumpluk, P. Simple Screening of Listeria monocytogenes Based on a Fluorescence Assay via a Laminated Lab-On-Paper Chip. Biosensors2017, 7, 56.
Pisamayarom K, Suriyasomboon A, Chaumpluk P. Simple Screening of Listeria monocytogenes Based on a Fluorescence Assay via a Laminated Lab-On-Paper Chip. Biosensors. 2017; 7(4):56.Chicago/Turabian Style
Pisamayarom, Kankanit; Suriyasomboon, Annop; Chaumpluk, Piyasak. 2017. "Simple Screening of Listeria monocytogenes Based on a Fluorescence Assay via a Laminated Lab-On-Paper Chip." Biosensors 7, no. 4: 56.
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Policy, Procedures, Guidance
- Docket No. FSIS 2013-0038 | PDF (Jun 11, 2015)
Best Practices Guidance for Controlling Listeria monocytogenes in Retail Delicatessens
- Video: Listeria Controls at Retail
FSIS hosted webinar held on September 29, 2015 with representatives from FSIS and the Centers for Disease Control and Prevention.
- Docket No. 04-032N | PDF (Dec 2, 2004)
- Docket No. 97-031FE | PDF (Dec 2, 2004)
Control of Listeria monocytogenes in Ready-to-Eat Meat and Poultry Products. Interim final rule; extension of comment period.
- FSIS Directive 10,240.4 - Revision 3 (Jan 10, 2014; PDF Only)
Verification Activities for the Listeria monocytogenes (Lm) Regulation and the Ready-to-Eat (RTE) Sampling Program
- FSIS Directive 10,240.5 - Revision 3 (Mar 28, 2013; PDF only)
Verification Procedures for Enforcement, Investigations and Analysis Officers (EIAOs) for the Listeria monocytogenes (Lm) Regulation and Routine Risk-Based Listeria monocytogenes (RLm) Sampling Program
- FSIS Directive 10,300.1 - Revision 1
Intensified Verification Testing (IVT) Protocol for Sampling of Product, Food Contact Surfaces and Environmental Surfaces for Listeria Monocytogenes (Mar 28, 2013; PDF Only)
- Compliance Guidelines
These guidelines help establishments producing Ready-to-Eat (RTE) meat and poultry products in their use of control methods for L. monocytogenes.
FSIS Recalls and Public Health Alerts
All FSIS recall information, including reports of recalls for possible Listeria monocytogenes contamination, is available in the Recalls section.
Provides FSIS risk assessment reports and models for Bovine Spongiform Encephalopathy, Clostridium perfringens, E. coli O157:H7, Listeria monocytogenes, Salmonella, etc.